The histone H4 lysine 20 monomethyl mark, set by PR-Set7 and stabilized by L(3)mbt, is necessary for proper interphase chromatin organization.
Drosophila PR-Set7 or SET8 is a histone methyltransferase that specifically monomethylates histone H4 lysine 20 (H4K20).L(3)MBT has been identified as a reader of methylated H4K20.It contains several conserved domains including three MBT repeats binding mono- and dimethylated H4K20 peptides.We find that the depletion of PR-Set7 blocks de novo H4K20